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operetta cls high content imaging system  (Revvity)


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    Structured Review

    Revvity operetta cls high content imaging system
    Operetta Cls High Content Imaging System, supplied by Revvity, used in various techniques. Bioz Stars score: 96/100, based on 3435 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/operetta+high+content+imaging+system/Operetta+CLS+high-content+analysis+system/10__1038_slash_s41598___026___73623___3-157-38-43
    Average 96 stars, based on 3435 article reviews
    operetta cls high content imaging system - by Bioz Stars, 2026-11
    96/100 stars

    Images

    Related Articles

    Live Cell Imaging:

    Article Title: Protocol for high-throughput identification of genes involved in pyroptotic cell death by live microscopy.
    Article Snippet: .. Alternatives: Several alternative systems could perform in-incubator fluorescent live-cell imaging, e.g., Invitrogen EVOS M7000 Imaging System, Axion Biosystems Omni Pro 12, ECHO Cellcyte 1, or Perkin Elmer Operetta High-Content Imaging System. .. Optional: To facilitate work in 384-well plate format, we suggest to pipette transfection reagent and seed cells with Multidrop Combi Reagent Dispenser (ThermoFisher Scientific).

    Imaging:

    Article Title: Protocol for high-throughput identification of genes involved in pyroptotic cell death by live microscopy.
    Article Snippet: .. Alternatives: Several alternative systems could perform in-incubator fluorescent live-cell imaging, e.g., Invitrogen EVOS M7000 Imaging System, Axion Biosystems Omni Pro 12, ECHO Cellcyte 1, or Perkin Elmer Operetta High-Content Imaging System. .. Optional: To facilitate work in 384-well plate format, we suggest to pipette transfection reagent and seed cells with Multidrop Combi Reagent Dispenser (ThermoFisher Scientific).

    Article Title: PEGylation modulates the pulmonary behaviour and antibacterial efficacy of tobramycin liposomes in advanced in vitro lung models.
    Article Snippet: The cytoplasm was stained using CellTracker Deep Red Dye (Thermo Fisher Scientific, C34565, Waltham, MA, USA) at 1× working concentration (excitation/emission: 630/660 nm), and nuclei were stained with Hoechst 33342 (Thermo Fisher Scientific, 62249, Waltham, MA, USA) at 2 μM (excitation/emission: 350/461 nm), according to the manufacturers’ instructions. .. Images were acquired at 40× magnification using an Operetta high-content imaging system (Revvity, Waltham, MA, USA). ..

    Article Title: Novel Cell Cycle Inhibitors Decrease Primary and Metastatic Breast Cancer Growth In Vivo.
    Article Snippet: Subsequently, 20 μL of D-PBS (Invitrogen, Cat#14190169) or dye mix (final concentrations of 4 μM Hoechst (Invitrogen, H21486) and 4 μM TOTO-3 (Invitrogen, Cat# T3604)) was added, and cells were stained for one hour at 37 ◦C. .. Plates were scanned using an Operetta High-Content Imaging System (Perkin Elmer, Waltham, MA, USA), and CTC cluster analysis was performed using Columbus Image Data Storage and Analysis System (Perkin Elmer). ..

    Article Title: Omicron SARS-CoV-2 shows NRP1-linked susceptibility in adipose-derived MSCs and alters primitive cord blood CD34⁺ HSPCs
    Article Snippet: The nuclei of the cells were stained using Hoechst dye obtained from Thermo Fisher Scientific. .. The Operetta high-content imaging system (PerkinElmer) was used to detect and analyze the fluorescent signals. .. Automated quantification of infected cells was performed using the Harmony software (PerkinElmer), which was set to analyze 13 images per well.

    Article Title: The malate-aspartate shuttle supports thermogenic lipid mobilization in brown adipocytes.
    Article Snippet: After the assay, cells were fixed using 4% paraformaldehyde (Thermo Fisher Scientific, Roskilde, Denmark). .. To normalize the data for possible differences in cell number, nuclei were stained with 1 lg mL 1 Hoechst 33342 (Thermo Fisher Scientific, Roskilde, Denmark) and nuclei were counted using the Operetta High-Content Imaging System (PerkinElmer, Waltham, MA). .. Super-resolution live-cell imaging was performed on a Zeiss LSM880 using a 639 Plan-Apochromat oil-immersion lens and AiryScan super-resolution detector with a humidified 5% CO2 chamber on a temperature-controlled stage (37 °C).

    Article Title: Tomatidine Exposure During Early Myogenesis Promotes Hypertrophy of C2C12 Muscle Cells Through Increased Nuclei Accretion
    Article Snippet: After treatments were refreshed on D2, all wells without cells were filled with PBS, and the plate sealed with a gas permeable film (Axygen, Corning, NY, USA) followed by the plate lid. .. Cells were placed into the Perkin Elmer Operetta High Content Imaging system at 37 o C in 5% CO 2 for 46 h. Images were captured every hour from 33 pre‐defined locations within each well, using a 10× LWD objective. ..

    Staining:

    Article Title: The malate-aspartate shuttle supports thermogenic lipid mobilization in brown adipocytes.
    Article Snippet: After the assay, cells were fixed using 4% paraformaldehyde (Thermo Fisher Scientific, Roskilde, Denmark). .. To normalize the data for possible differences in cell number, nuclei were stained with 1 lg mL 1 Hoechst 33342 (Thermo Fisher Scientific, Roskilde, Denmark) and nuclei were counted using the Operetta High-Content Imaging System (PerkinElmer, Waltham, MA). .. Super-resolution live-cell imaging was performed on a Zeiss LSM880 using a 639 Plan-Apochromat oil-immersion lens and AiryScan super-resolution detector with a humidified 5% CO2 chamber on a temperature-controlled stage (37 °C).

    Saline:

    Article Title: Neuroprotective eremophilane sesquiterpenoids from Ligularia species attenuate glutamate-induced oxidative stress via MAPK signaling.
    Article Snippet: .. Cells were washed twice with Dulbecco's phosphate-buffered saline (DPBS), and images were captured using the Operetta High-Content Imaging System (PerkinElmer). ..

    High Content Screening:

    Article Title: Neuroprotective eremophilane sesquiterpenoids from Ligularia species attenuate glutamate-induced oxidative stress via MAPK signaling.
    Article Snippet: .. Cells were washed twice with Dulbecco's phosphate-buffered saline (DPBS), and images were captured using the Operetta High-Content Imaging System (PerkinElmer). ..

    Article Title: Allele-resolved monosome and polysome sequencing identifies cis-variants affecting mRNA translation.
    Article Snippet: .. GFP and mCherry signals, and digital phase contrast were detected at 24, 36, and 48 h post-transfection using Operetta High Content Imaging System (PerkinElmer). ..

    Phase Contrast:

    Article Title: Allele-resolved monosome and polysome sequencing identifies cis-variants affecting mRNA translation.
    Article Snippet: .. GFP and mCherry signals, and digital phase contrast were detected at 24, 36, and 48 h post-transfection using Operetta High Content Imaging System (PerkinElmer). ..



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    Image Search Results


    (A) Detection of SARS-CoV-2 infected cells using high-content imaging under control conditions. Fluorescent signals: green (anti-SARS-CoV NP mAb), blue (Hoechst). (B) Representative flow cytometry analysis plots showing NP+ cells. (C) Measurement of fluorescent intensity in NP+ cells. (D) Percentage of infected MSCs. (E) The supernatants of infected MSCs were collected and titrated on Vero-E6/TMPRSS2 cells to measure infectious viral particle production using the focus-forming assay. (F) After RNA extraction from infected MSCs, the expression of IDO1 was examined using real-time PCR. The graph displays the expression of IDO1 normalized to GAPDH , with the mock group serving as the control. Data are represented as means ± SEM from three independent biological replicates. For , the Delta condition was available from only two independent donors and was therefore presented descriptively and excluded from the statistical analysis. Statistical comparisons among the remaining groups were performed using one-way ANOVA followed by Tukey’s multiple-comparisons test. One-way ANOVA showed a significant overall difference among groups, F(2, 6) = 6.794, P = 0.0287. * P < 0.05, ** P < 0.01, *** P = 0.0001, **** P < 0.0001.

    Journal: PLOS One

    Article Title: Omicron SARS-CoV-2 shows NRP1-linked susceptibility in adipose-derived MSCs and alters primitive cord blood CD34⁺ HSPCs

    doi: 10.1371/journal.pone.0360439

    Figure Lengend Snippet: (A) Detection of SARS-CoV-2 infected cells using high-content imaging under control conditions. Fluorescent signals: green (anti-SARS-CoV NP mAb), blue (Hoechst). (B) Representative flow cytometry analysis plots showing NP+ cells. (C) Measurement of fluorescent intensity in NP+ cells. (D) Percentage of infected MSCs. (E) The supernatants of infected MSCs were collected and titrated on Vero-E6/TMPRSS2 cells to measure infectious viral particle production using the focus-forming assay. (F) After RNA extraction from infected MSCs, the expression of IDO1 was examined using real-time PCR. The graph displays the expression of IDO1 normalized to GAPDH , with the mock group serving as the control. Data are represented as means ± SEM from three independent biological replicates. For , the Delta condition was available from only two independent donors and was therefore presented descriptively and excluded from the statistical analysis. Statistical comparisons among the remaining groups were performed using one-way ANOVA followed by Tukey’s multiple-comparisons test. One-way ANOVA showed a significant overall difference among groups, F(2, 6) = 6.794, P = 0.0287. * P < 0.05, ** P < 0.01, *** P = 0.0001, **** P < 0.0001.

    Article Snippet: The Operetta high-content imaging system (PerkinElmer) was used to detect and analyze the fluorescent signals.

    Techniques: Infection, Imaging, Control, Flow Cytometry, Analysis, Focus Forming Assay, RNA Extraction, Expressing, Real-time Polymerase Chain Reaction

    (A) Representative western blot depicting the expression of NRP1 in AD-MSCs (No siRNA), cells transfected with non-targeting siRNA (siControl), or siNRP1 for 24 hours. β-actin was used as a loading control. (B) Graph displaying the quantification of NRP1 normalized to β-actin (n = 3). This experiment represents three independent biological replications. Statistical analysis was performed using one-way ANOVA followed by Tukey’s multiple comparisons test. (C) Transfected cells were visualized using high-content imaging. Fluorescent signals: green for anti-NRP1 mAb, blue for Hoechst. (D) Percentage of NRP1-expressing cells normalized to cells transfected with siControl. Data are presented as mean ± SEM from three independent experiments. Statistical analysis was conducted using a paired t-test. Next, siRNA-transfected cells were infected with different variants of SARS-CoV-2. (E) SARS-CoV-2-infected cells were detected using high-content imaging. Fluorescent signals: green for anti-SARS-CoV NP mAb, blue for Hoechst. (F) The fluorescent intensity of NP+ cells was measured. All data represent three independent experiments. Statistical analysis among each group of variant was performed using an unpaired t-test. * P < 0.05, ** P < 0.01, *** P = 0.0001, **** P < 0.0001.

    Journal: PLOS One

    Article Title: Omicron SARS-CoV-2 shows NRP1-linked susceptibility in adipose-derived MSCs and alters primitive cord blood CD34⁺ HSPCs

    doi: 10.1371/journal.pone.0360439

    Figure Lengend Snippet: (A) Representative western blot depicting the expression of NRP1 in AD-MSCs (No siRNA), cells transfected with non-targeting siRNA (siControl), or siNRP1 for 24 hours. β-actin was used as a loading control. (B) Graph displaying the quantification of NRP1 normalized to β-actin (n = 3). This experiment represents three independent biological replications. Statistical analysis was performed using one-way ANOVA followed by Tukey’s multiple comparisons test. (C) Transfected cells were visualized using high-content imaging. Fluorescent signals: green for anti-NRP1 mAb, blue for Hoechst. (D) Percentage of NRP1-expressing cells normalized to cells transfected with siControl. Data are presented as mean ± SEM from three independent experiments. Statistical analysis was conducted using a paired t-test. Next, siRNA-transfected cells were infected with different variants of SARS-CoV-2. (E) SARS-CoV-2-infected cells were detected using high-content imaging. Fluorescent signals: green for anti-SARS-CoV NP mAb, blue for Hoechst. (F) The fluorescent intensity of NP+ cells was measured. All data represent three independent experiments. Statistical analysis among each group of variant was performed using an unpaired t-test. * P < 0.05, ** P < 0.01, *** P = 0.0001, **** P < 0.0001.

    Article Snippet: The Operetta high-content imaging system (PerkinElmer) was used to detect and analyze the fluorescent signals.

    Techniques: Western Blot, Expressing, Transfection, Control, Analysis, Imaging, Infection, Variant Assay